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Banda Aceh, Aceh, Indonesia 23111

Inhibition of α-Glucosidase Activity and The Toxicity of Tristaniopsis merguensis Griff. Leaf Extract

Authors

  • Robby Gus Mahardika Department of Chemistry, Universitas of Bangka Belitung
  • Occa Roanisca Department of Chemistry, Faculty of Engineering, University of Bangka Belitung, Bangka Belitung, Indonesia
  • Fajar Indah Puspita Sari Department of Chemistry, Faculty of Engineering, University of Bangka Belitung, Bangka Belitung, Indonesia, fipuspitas@gmail.com

DOI:

https://doi.org/10.22373/ekw.v6i1.5732

Keywords:

Inhibition of ?-glucosidase, acetone extract, Tristaniopsis merguensis Griff., Brine Shrimp Lethality Test, BSLT

Abstract

Abstract : This study aims to determine the antidiabetic activity and toxicity of the acetone extract of Tristaniopsis merguensis Griff leaf. The antidiabetic test was the α-glucosidase inhibition method, while the toxicity test used the Brine Shrimp Lethality Test (BSLT) method. The acetone extract possessed antidiabetic activity with an IC50 value of 8.83 ± 0.31 (μg/mL). This value is not much different from the positive control of quercetin which has an IC50 value of 6.04 ± 0.14 (μg/mL). The characteristics of the FT-IR spectrum of acetone extract showed that Tristaniopsis merguensis leaf has the groups Ar-OH (phenolic), -OH (hydroxyl), C=O (ketone) and C=C (aromatic). Based on the toxicity test, the Tristaniopsis merguensis leaf acetone extract has an LC50 value of 959.25 ppm which means that the acetone extract is toxic. Therefore, the acetone extract of Tristaniopsis merguensis might be the potential agent of antidiabetic.

Abstrak : Penelitian ini bertujuan untuk mengetahui aktivitas antidiabetes dan toksisitas dari ekstrak aseton daun Tristaniopsis merguensis Griff. Uji aktivitas antidiabetes ditentukan berdasarkan metode inhibisi enzim α-glucosidase, sedangkan toksisitas ditentukan berdasarkan metode Brine Shrimp Lethality Test (BSLT). Ekstrak aseton memiliki aktivitas antidiabetes dengan nilai IC50 8,83 ± 0,31 (μg/mL). Nilai ini tidak jauh berbeda dengan kontrol positif quersetin yang memiliki nilai IC50 6,04 ± 0,14 (μg/mL). Karakteristik spektrum FT-IR ekstrak aseton menunjukkan bahwa daun Tristaniopsis merguensis memiliki gugus fungsi Ar-OH (fenolik), -OH (hidroksil), C=O (keton) dan C=C (aromatik). Berdasarkan uji toksisitas, ekstrak aseton daun Tristaniopsis merguensis memiliki nilai LC50 sebesar 959,25 ppm yang berarti bahwa ekstrak aseton bersifat toksik. Oleh karena itu, ekstrak aseton dari Tristaniopsis merguensis berpotensi untuk dijadikan agen antidiabetes.

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Published

2020-06-30

Issue

Vol. 6 No. 1 (2020)

Section

Articles

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